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At 48 h after plating, the medium was replaced with DMEM supplemented with 5% horse serum (Sigma), 20 mM D-glucose and, on day 4, cytosine arabinoside (10 M) to prevent non-neuronal proliferation 33

The pellets were washed once with M9 buffer, transferred to 1.5 mL microcentrifuge tubes, flash-frozen in liquid nitrogen, and stored at 80 C

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This study aims to examine ferroptosis as a potentially critical pathway in effective targeting of thyroid cancer (TC) cells

However, several groups are at high risk of deficiency and may benefit significantly from regular injections or supplementation