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This process is morphologically marked by small mitochondria with condensed membrane densities, and biochemically by iron overload and GSH depletion, leading to loss of antioxidant defense and irreversible oxidative damage ( 3.2 Key molecular drivers of ferroptosis Iron Overload: Excess intracellular ferrous iron (Fe 2+ ) catalyzes the Fenton reaction, producing hydroxyl radicals (OH) that initiate lipid peroxidation cascades

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Significant co-expression changes in aminoacyl-tRNA biosynthesis metabolic genes were observed in CM5 and CM7, while the co-expression of amino acid biosynthesis genes increased notably in CM5-CM9, particularly approaching 6 h after treatment (Supplementary Fig

Key Takeaway: Dosage is not a "one size fits all" calculation