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Description

Hypokalemia and sudden death may occur in severe megaloblastic anemia which is treated intensely with vitamin B 12

For CCL2 knockdown experiments, HepG2 cells were plated at 1 10 5 cells per well of a 24-well plate and transfected with 20 nM of siRNA against CCL2 (siCCL2, CCL2 Human ON-TARGETplus siRNA, Dharmacon, L-007831-00-000) using Lipofectamine RNAiMAX (Invitrogen, 13778150) in Opti-MEM reduced-serum medium (Gibco) in accordance with the manufacturers protocol