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In brief, bones were fully decalcified in 14% EDTA at a pH of 7.4 for 2 weeks before embedding in optimal cutting temperature mounting media (Fisher HealthCare, 23-730-571) and sectioning at 50 m on a cryostat (Leica)

doi: 10.1186/s12906-017-1915-9 257 PratheeshkumarPKuttanG

The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells

For example, monoclonal antibodies to be used may be made by the hybridoma method first described by Koehler et al., Nature, 256: 495 (1975), or may be made by recombinant DNA methods (see, e.g., U.S