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In subunit 2 the domain I helices are dark cyan -strands are orange, and the domain II helices are light cyan

Using reduced and oxidized glutathione, you can refold this protein into a more compact and more energetically favorable protein on the right where the disulfide bond is now between the first and third cysteine residues Glutathione is important in maintaining redox balance in cultured cells, and is already included in many formulations of cell media (Ishii & Mann, 2014)

The transfected cells were then cultured in medium containing puromycin (5 g ml 1 ) for 10 days

Material and methods : The study was a multicenter, pragmatic, stepped-wedge cluster-randomized trial

A committee responsible for the justification of whether the event is considered VOC or not was formed by the investigators (Fatma Ebeid, Sara Mekkya, Nihal Hussien)