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The next day, slides were washed with TBST, followed by staining with the secondary antibodies of goat anti-rat IgG conjugated with TRITC (Jackson Lab) and goat anti-mouse IgG conjugated with FITC

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Purification Protein A column, followed by peptide affinity purification Immunogen KLH conjugated synthetic peptide between 387-416 amino acids from the C-terminal region of human Glutathione reductase