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Abnormal MAO activity disrupts this equilibrium, triggering a cascade of depressive symptoms [160]

3.3 Methionine restriction exerts its antitumor effects by polyamine metabolism inhibition and metabolic interference The high demand for methionine in cancer cells is sustained in vivo not only from dietary sources but also from limited methionine cycling and salvage pathways, namely remethylation and the MTA salvage pathway

While transcriptomic studies have highlighted shifts in lysosomal activity, redox balance, and lipid metabolism with increased passage number 17 , comprehensive mechanistic analyses linking these genetic changes to metabolic flux alterations remain lacking