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thus, the uptake, distribution, and elimination of Cu are tightly regulated

It serves as a functional cofactor for lysyl oxidase (the enzyme that cross-links collagen fibers properly) and for superoxide dismutase (SOD1, a key antioxidant enzyme)

Aliquots of reaction mixtures containing in vitro -translated proteins were added to Ni-NTA agarose beads coupled to 4 g of recombinant N-6His RCC1 (negative control) or recombinant N-6His GSTP1