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Briefly, cortices were digested with 1 mg/ml collagenase/dispase and 2.5 g/ml DNAse (Roche) in dissection buffer (HBSS, 10 mM HEPES, 0.5% BSA, 5000 U/ml penicillin/streptomycin) for 45 min at 37 C

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All the conditions that until now sat largely in the grey market