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Total proteins from lysed cells or patient tissue were extracted using RIPA lysis buffer and electrophoresed by SDS-PAGE and transferred onto PVDF membranes subsequently

While steady state plasma concentrations of niacin are generally higher in women than in men, the absorption, metabolism, and excretion of niacin appears to be similar in both genders

Ethics declarations Conflict of interest This research has no known conflict of financial interests or personal relationships that could have appeared to influence the work reported in this paper