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Description

Figure 2 For experiment 3: fixed dose study of Atlas987 combined with paroxetine

Following centrifugation (800 g , 5 min), cell pellets were washed twice with sorting buffer (1 PBS, without Ca 2+ or Mg 2+ , containing: 1 mM Na-EDTA, 25 mM HEPES (pH 7.0), 1% (v/v) FBS and 100 U/ml penicillin-streptomycin) and resuspended with ~200 L of buffer containing DAPI to exclude dead cells from analysis