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For histological analysis, the femoral and tibial condyles were fixed in 10% neutral buffered formalin, decalcified with 20% EDTA, embedded in paraffin, and sectioned into 5-m thick frontal slices

After amplification, PCR products were electrophoresed on agarose gels and transferred onto nylon membranes using Southern blotting techniques

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It functions as a metabolic modulator by inhibiting the NNMT enzyme, rather than acting as a signaling peptide

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