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We use a very fine needle, so the entire process is over in a few seconds

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Global analysis of gene expression and Gene Set Enrichment Analysis (GSEA) in gastrocnemius muscle revealed that expression of pro-inflammatory cytokines and chemokines which are typically increased in obese state 22,25,26,27,28 such as chemokine (C-C motif) ligands 2, 6, 7, 9, 11 ( Ccl2 (Mcp1 ), Ccl6, Ccl7 (Mcp3 ), Ccl9, Ccl11 ) and chemokine (C-X-C motif) ligands 1, 9, 12 ( Cxcl1, Cxcl9, Cxcl12 ), and their receptors, IL6ra, Ccr1, Ccr2, Ccr3 , and Cxcr4 was not changed in muscle tissue of Cpt1b m/ mice compared to Cpt1b fl/fl mice (Supplementary Table S1)

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