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hippocampal CA3 synapse loss, suppressed neurogenesis and chronic-stress BDNF reduction

Such an indication is unique from most other drugs and compounds identified by the NIA-supported Intervention Testing Program (ITP) to improve lifespan, as acarbose, 17--estradiol, nondihydroguaiaretic acid (NDGA), and protandim, all preferentially improve male lifespan 35,36

ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability

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The incision is so tiny that there is no need for any sutures