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21,22 Representative AMP sequences from different sources are shown in Table 1

All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution Lane 1: Rat testis tissue lysate at 20 g Lane 2: Mouse testis tissue lysate at 20 g Lane 3: Human testis tissue lysate at 20 g Secondary All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution Predicted band size: 22 kDa Observed band size: 19 kDa,22 kDa false Exposure time: 1s WB Lab Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066) Lanes 1 - 3 : Merged signal (red and green)

The total amino acid (TAA) composition of human milk, including protein-bound AAs and FAAs, is used to evaluate the quantity and the quality of the milk proteins and hence is well characterized ( FAAs in human milk have been reported to account for ~510% of the TAA content (47)