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After the solution had entered the column, 550 l of 100 mM HEPES p H 7.0 were loaded on the column and 300 l of 100 mM HEPES p H 7.0 were used to elute the protein

10.1016/j.yexcr.2014.07.037 55 KimS

hGH indirectly controls growth and protein synthesis by stimulating the liver and other tissues to produce a group of proteins called insulin-like growth factors (IGFs)

When using liposomes containing cationic lipids, the dried lipid film is resuspended in an appropriate solution such as sterile water or an isotonic buffer solution such as 10 mM Tris/NaCl, sonicated, and then the preformed liposomes are mixed directly with the DNA