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10.1038/tp.2016.212 39 MenniniT.GobbiM

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The NADPH produced via the PPP is required for the production of glutathione (GSH) ( in vitro GSH detection assays also revealed an accumulation of GSH in melatonin treated cells that was not found in vehicle controls

Cell culture and treatment MRC-5 cells were cultured in minimum essential medium (Gibco) with 10% fetal calf serum (Gibco) and 1% penicillinstreptomycin