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At 48 h after plating, the medium was replaced with DMEM supplemented with 5% horse serum (Sigma), 20 mM D-glucose and, on day 4, cytosine arabinoside (10 M) to prevent non-neuronal proliferation 33

Smooth muscle cells and the formation, degeneration, and rupture of saccular intracranial aneurysm wall--a review of current pathophysiological knowledge

Spine 1 , 1003 (2016)

And you have to do all of this work in advance of applying. That could easily mean a developer sinks $100,000 into a LIHTC project before even applying for the tax credit which could get rejected