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Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)

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They not only directly activate the expression of GAD65/67 and VGAT, ensuring the GABAergic properties of interneurons, but also regulate genes such as Arx, guiding interneurons to migrate from the ventral forebrain to the cortex and form inhibitory circuits