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Animals were housed in the animal facility of Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, four per cage, under controlled environmental conditions with day/night cycles of 12 h, temperature 221C and relative humidity 50-60% with food and tap water supplied ad libitum

Frequency of Injections Regular, scheduled injections as part of a treatment plan may lead to more consistent and noticeable benefits

Every order includes a certificate of analysis documenting purity verification results

Functional deficiency are the biochemical changes such as elevated homocysteine or methylmalonic acid levels