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For ex vivo tracing experiments, CD4 + T cells isolated from untreated mice were further cultured for 2 h in diluted medium with C 13 -labelled BHB

Conversely, the level of TOS was significantly increased in the MTC group when compared to both the control group and the group with benign nodules

For preparation of frozen stocks, the organoid cultures were dissociated, mixed with Recovery cell culture freezing medium (Gibco) and frozen following standard procedures

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The study demonstrated that YSTJ administration significantly improved clinical symptoms, enhanced sciatic nerve conduction velocity (NCV), and reduced sciatic nerve demyelination