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Although MRI provides superior soft-tissue contrast for complex cases, real-time ultrasound outperforms MRI in terms of dynamic assessment, guiding precision interventions through simultaneous vascular and structural evaluation 7,9

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The DBA/2J mouse strain, which develops a similar phenotype of chronic glaucoma, has mutations in the Gpnmb gene (encoding a lysosomal-related glycosylated protein) and the Tyrp1 gene (encoding a melanosomal protein with possible enzymatic and structural functions) (38, 39)

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The mice were injected intravenously with PBS, Ru(II)-OH, NP2, NP4 (2 mg/kg Ru) on days 1, 4, 7, 10, respectively