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CRISPRi cell line generation and validation K562 sgNT and K562 sg NPC1 cell lines were generated using Cas9sgRNA ribonuclear protein transfection (sg NPC1 sgRNA sequence: GGACGATCCTTGGCTTGGAC) as previously described 26 , and knockout was validated by western blot (Extended Data Fig

Cerwenka A, Baron JL, Lanier LL

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Four to six weeks is one of the most common KLOW cycle ranges discussed in research communities

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