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Description

Daijo K, Kawaoka T, Nakahara T, Nagaoki Y, Tsuge M, Hiramatsu A, Imamura M, Kawakami Y, Aikata H, Hara K, Tajima G, Kobayashi M, Chayama K

C ,

10.22034/gjesm.2017.03.02.005 74 JinP.ZhangJ.WanJ.OvermansS.GaoG.YeM.et al

In an assay mixture 100 g protein was incubated in a 1 ml solution containing 5 mM manganese chloride or MnCl 2 , 0.1 mm DTT, 0.1 mM p-coumaric acid, 0.05 mM NADP(H), 30 mM sodium acetate buffer (pH 7.5) and incubated under dark