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for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein

Importantly, GHK itself is part of type I collagen

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Solution left out of the fridge Short excursions at room temperature (under 2 hours) are generally tolerable