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Thin walled filamented borosilicate glass pipettes (World Precision Instruments) were pulled to the desired tip diameter ( ~ 1 m

Treatments are gentle and well-tolerated

You may also develop more serious, long-term health concerns

Then, promoter #14 and SOD were amplified from pKLH167 and ligated into the backbone of pKLH337, yielding pKLH341

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