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The standard curve was drawn against the standard samples, and the relative glutamate, glycine and cysteine level in the samples was calculated by the protein concentration of the samples

For PNGase H+ application, exchanging with distilled water was omitted, and slides were immediately dried in a desiccator after antigen retrieval to ensure acidic conditions necessary for optimal PNGaseH+ activity

IL-10 is a crucial cytokine that maintains immune homeostasis