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Additionally, the long-term effects of nasal glutathione administration are still under investigation, and more research is needed to fully understand its impact.

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In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of gammaglutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 4)

Wash the cells with 300 l of PBS and centrifuge at 200 x g for 10 minutes at 4C

Together, these findings support a consistent role for IL-24 in driving type 2 inflammation and airway remodeling across both OVA and HDM models, highlighting its potential as a therapeutic target in allergic asthma