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Briefly, the pancreatic duct was cannulated and distended with 4 C collagenase/protease solution using Collagenase P (Sigma-Aldrich, USA) in 1x Hanks balanced salt solution and 0.02% bovine serum albumin

doi: 10.1182/blood-2014-10-608133 82 MussaiFWheatRSarrouEBoothSStavrouVFultangLet al

Front Aging Neurosci 17

For COS-7 cells, cultures were washed with PBS-CM and uptake reactions were initiated by addition of either L-H-glutamate (50 nM final concentration) or a mixture of unlabeled L-glutamate and L- 3 H-glutamate (150 M, final concentration, 99 % unlabeled and 1% labeled)

Rather its properties are most supportive when taken only when needed and as soon as possible at the first sign of feeling rundown due to occasional stress