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In addition, infected cells were selected by using 2.5 g/ml puromycin (MCE, USA) for 14 days, and the transfection efficiency was verified by RT-qPCR and Western blot analysis

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For each sample, 25 l of liposomes were mixed with 25 l of the 2 x reaction buffer consisting of 10LM [ 3 H]GSSG (Figure 3a and Extended Data Figure 4l) or 40 M [ 3 H]GSSG (Extended Data Figure 7F), 150 mM KCl, and 50 mM HEPES (pH 7.4)

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If difficult or painful intravenous access has you or a loved one stressing over medical procedures, there is a solution the butterfly needle