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Cell debris were removed by centrifugation at 500 g for 5 min, and mitochondria were pelleted by centrifugation at 20,000 g for 20 min

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Keelan JA, Marvin KW, Sato TA, Coleman M, McCowan LM, Mitchell MD: Cytokine abundance in placental tissues: evidence of inflammatory activation in gestational membranes with term and preterm parturition

(A) Intracellular concentrations of glucose and (B) reduced and oxidized nicotinamide adenine nucleotide and (D) L-lactate in Salmonella grown aerobically in MOPS-GLC minimum medium, pH 7.2 to an OD600 of 0.25 at 37C