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doi: 10.4103/0972-2327.99993

The next day, medium was changed to differentiation conditions (DMEM + 2% horse serum + 1% ABAM) for 4 days to induce myotube formation

doi: 10.1016/0041-008x(89)90283-4

In this study, glutamine flux was directed toward the generation of R-2-HG in IDH1/2 mutant cells, and the kinetics of R-2-HG formation were proportionally of the same order of magnitude as those of glutamate or -KG formation via glutaminolysis

In parallel, a study in PCOS women with hyperhomocysteinemia found that 1 mg folic acid daily for 3 months effectively decreased Hcy levels in all participants, with a more significant diminution observed in non-IR women, implying that folic acid supplementation contributes to improving the bodys methylation status and mitigates associated vascular endothelial damage (66)