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Furthermore, several more recent studies using the same primary neuronal culture preparation protocol as the earlier study found either no difference between synaptic and extrasynaptic NMDA receptors in promoting excitotoxicity (reviewed in Papouin and Oliet, 2014) or found that both receptors were required for cell death (Zhou et al., 2013)

A Bradford assay (Thermo Scientific) was used to determine the total protein concentration, with ovalbumin as the protein standard

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