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The simulated magnitude of the interaction (AUCR, 1.02) with midazolam was ~30% lower than observed (AUCR, 1.39) if only reversible CYP3A inhibition was considered, indicating that time-dependent inhibition contributes to the mechanism of the interaction

Notably, incubation of AX with L-cysteine (with the nonprotected-amino group) did not afford DKP, but mainly, the product corresponding to the aminolysis of the -lactam ring, which remained conjugated to the amino group of cysteine through an amide bond ( Figure S8 )

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Wolff et al., 2023)