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Borkent, J., Ioannou, M., Laman, J

reverse primer: 5-CACCGTCAATGTAGTAAGGCA-3) and the primers for -Actin as control (forward primer: 5-CGTTCCTGGGTATGGAATCG-3

Beads were extensively washed with lysis buffer, and bound proteins were eluted with 250 mM imidazole, followed by overnight incubation with PreScission protease to remove the 6His-tag