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Senescence-associated -galactosidase (SA--gal) activity was measured in both cells and frozen tissues using a commercial kit, providing a visual and quantitative indicator of senescent cells

Chronic exposure to these drugs depletes presynaptic monoamine stores, impairs long-term neuronal signaling, and induces neurodegeneration ( 4.2 Oxidative stress and mitochondrial dysfunction The excessive release of monoamines promotes ROS formation through the auto-oxidation of dopamine and norepinephrine

Various phosphospecific antibodies were used in the IHC analysis to provide both qualitative and quantitative information of the signalling pathways in the tumours (Figure 4)

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