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The Role of Glutathione in Detoxification In addition to its antioxidant properties, glutathione is essential for detoxification

It is worth noting that many of the top ATF4-dependent genes that scored as being induced by the ISR alone showed some degree of rapamycin-sensitive induction with insulin but did not reach statistical significance in the RNA-seq analyses

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Downstream effects via active metabolites, gene-expression changes, and enkephalinase inhibition persist for hours to days, which is why dosing frequency is set by downstream windows rather than plasma kinetics