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Moreover, other groups reported that the Asp-248, Glu-25, Asn-127, and Asp-40 residues in SLC30A10 are required for its ability to export Mn when expressed in HeLa cells 91 , whereas the Mn transport activity of SLC30A10 with an Asn-43 mutation was similar to that of wild-type SLC30A10 when expressed in various cell types, including hepatic HepG2 cells, neuronal AF5 cells, HEK293 cells, and embryonic fibroblasts 92