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Interestingly, the analysis of the tumors by immunoblot revealed decreased levels of galectin-1 and a subsequent elevated levels of granzyme B in tumors that were treated with the combination of sertraline and irradiation ( p = 0.047, p = 0.016, respectively) (Fig

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The protein supernatant was collected by centrifugation at 15,000 rpm at 4 for 10 min and the protein concentrations were measured by bicinchoninic acid (BCA) protein assay kit (Beyotime, China)

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