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Next, the lipids were rehydrated in buffer containing 150mM KCl, 50 mM HEPES buffer (pH 7.4) at a concentration of 8 mg/ml by shaking until fully dissolved, followed by 6 freeze-thaw cycles

Moreover, SN, in a concentration of 2 to 14 parts per million, gives the meat its desirable red color by forming nitrosylating compounds that latterly interacts with myoglobin of the meat producing the red color 3