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Results mTORC1 signaling activates a subset of ATF4-dependent genes also activated by the integrated stress response To identify the ATF4-dependent gene targets downstream of mTORC1, we compared the insulin-induced, rapamycin-sensitive transcripts between wild-type MEFs and those with biallelic loss of ATF4 via CRISPR/Cas9 gene deletion (see methods)

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The GSH levels of the BSO-treated cells quantified using our RT-based flow cytometry method is well correlated to the values measured using cell lysates with a glutathione reductase-based biochemical assay ( R 2 =0.99, Fig