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After reconstitution, refrigerate them at 28C and use within the recommended timeframe

Follow with moisturizer and SPF during the daytime to protect your results.

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For BrdU experiments, bone marrow cells were cultured into macrophages in 100 mm dishes for two or three days, respectively, and reseeded in 6-well plates [80,000 cells/cm ( Non-adherent cells were collected, adherent cells were washed in HEPES-buffered saline solution (Promocell, Heidelberg, Germany), detached by incubating in Accutase (Sigma Aldrich) and, together with non-adherent cells, resuspended in FACS buffer before flow cytometry analysis