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The cells were washed three times with PBS prior to being fixed in 4% paraformaldehyde at a temperature of 4C for a duration of 20 min

In Canada, as in much of the world, police and prosecutors use measurable blood alcohol concentration thresholds to legally prove that a driver was driving while impaired by alcohol

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Also, both mutant and WT SOD1 produce cytotoxic levels of H 2 O 2 via a cysteine redox regulation system (Bakavayev et al., G93A mice show significantly increased protein carbonyl levels in the spinal cord, including elevated carbonylation of mutant SOD1, which has been linked to motor neuron degeneration (Poon et al., 2005)