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Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C

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A peer-reviewed clinical paper confirmed that ADH released during dehydration can bind to uterine oxytocin receptors and stimulate contractions

Unlike most of the growth factors that are ready to function upon secretion, TGF- is unique in that it is secreted as part of a latent complex that is stored in the extracellular matrix (ECM)