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For immunofluorescence staining, cells were rinsed three times with PBS before being incubated with BODIPY TM 581/591 C11 dye in serum-free medium at 37 C with 5% CO2 for 30 min before being visualized under a fluorescence microscope (Evos FL, Thermo Fischer Scientific) with objective 40

A polymorphism or variation in the sequence within an ER-response element located in the SELENOS promoter was found to result in reduced SELENOS promoter activity and gene expression (36)

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Pretreatment of cultured preadipocytes with arachidonic acid during the differentiation phase without a cAMP-elevating agent enhances fat storage after the maturation phase