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MS identity confirmation not just HPLC purity is particularly important now because HPLC purity measures percentage of the dominant peak, but cannot confirm that the dominant peak is actually the correct peptide

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The column was washed twice by adding 500 L of wash solution and centrifuging at 3300 g for 2 min

Vitamin B12 deficiency reduces proliferation and promotes differentiation of neuroblastoma cells and up-regulates PP2A, proNGF, and TACE