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A single-cell mycobacterial suspension was prepared by washing bacteria collected by centrifugation twice with phosphate-buffered saline supplemented with 0.05% Tween-80 (PBST 80 ) and then centrifuging at 200 g for 10 min

Longer term this can also lead to damage to the joint and surrounding bone and cartilage

63 Also, digital twins have been employed in the industry since 2002 to enhance manufacturing processes and manage the entire product life cycle more effectively

Incorrect reconstitution can lead to peptide degradation, aggregation, loss of biological activity, and unreliable experimental results