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Pull back the plunger to the mark of the amount of water you plan to use (e.g., the 1mL or 2mL mark)

Glycerol release was measured under basal and forskolin-stimulated conditions using a colorimetric assay kit (Sigma-Aldrich)

The sensitivity of the U87vIII CHCHD2 WT and KO cells to a panel of cytotoxic agents was determined using the SRB assay according to previously published protocols (31)