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Even so, the SMT15-specific antibody was able to detect a 144 kDa SMT15 protein in membrane factions prepared from the wild-type and complemented smt15-1 pSMT15.1 #7 strains but not from the smt15-1 strain (Supplemental Figure S1B), confirming that SMT15 is a membrane protein (Fang et al., 2014)

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