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Cells were exposed to the experimental conditions for 6h and representative images of the formed tube-like structures were acquired on an Olympus IX53 Inverted Microscope and analyzed with ImageJ software (imagej.nih.gov/ij/)

Rich in antioxidants, coffee naturally contains niacin (vitamin B3), riboflavin (vitamin B2), potassium, magnesium and various phenolic compounds, among other nutrients

Lau and Tymianski, 2010), resulting in cell necrosis or apoptosis (Khr, 2006

Who is most at risk of having low B12 levels

In more severe cases of anemia, treatments like blood transfusions or erythropoiesis-stimulating agents may be required